3D SKELETAL MUSCLE CULTURE DEVICES
Advance your skeletal muscle research projects and implement new 3D cell culture technologies with our OMEGAMP 3D skeletal muscle device series!
To help you create human-relevant in vitro models, each culture chamber of OMEGAMP and OMEGA96-MP contains two built-in micropillars that serve as anchor points for the 3D muscle culture. Simply watch your cells self-assemble into a stable and mature skeletal muscle microtissue (see video below for an example). Once sufficiently matured, these 3D microtissues twitch and contract as you would expect from skeletal muscle.
As always, all devices come ready-to-go!
Compared to monolayer muscle cultures that are known to detach from the surface after a short period of time, 3D skeletal muscle tissues are stable for longer-term culture applications and mature more rapidly and more completely. Once sufficiently matured, these microtissues twitch and contract as you would expect from skeletal muscle. This increased physiological relevance of this system makes it ideal for the development of new in vitro assays and disease models of both muscle and neuromuscular disorders.
Use myogenic progenitor cells from primary, iPSC or immortalized sources! Simply resuspend the cells in an ECM-derived hydrogel mixture and seed them into the muscle culture chamber. After polymerization, the hydrogel-suspended myoblasts begin to differentiate and self-assemble into a 3D muscle microtissue using the two micropillars as anchors. Watch the self-assembly in the video below .
CHOOSE YOUR FORMAT
If you are just starting out, we recommend using the OMEGAMP with two experiments per device. Ready to perform more experiments? Then save money with the HTS-compatible OMEGA96-MP featuring 96 experiments per microplate! Are you interested in neuromuscular junctions? Then the OMEGANMJ microfluidic co-culture device for motoneurons and 3D muscle cultures might be right for you.
START RIGHT AWAY
We ship all devices of the OMEGAMP series completely ready-to-go so that you can focus on performing experiments without losing any time. All OMEGAMP devices feature two built-in micropillars per experimental unit - no need to pre-assemble the devices! And of course, the devices come sterile.
ACCESS CULTURES DIRECTLY
OMEGAMP devices have open-top chambers for full access to the cultures for electrophysiological stimulation or recording. The ability to examine the contractile and electrophysiological characteristics of mature skeletal muscle is incredibly advantageous in the context of fundamental as well as drug development research. In addition, the thin high-transmissive PDMS base allows for optical access for high quality imaging.
With the OMEGAMP, you can build next-generation skeletal muscle models, while increasing your data output and saving money! The OMEGAMP features two experiments per device and we are now offering a fully HTS-compatible microplate version: the OMEGA96-MP. This plate is equivalent to 12 OMEGAMP kits, at a fraction of the price.
3D SKELETAL MUSCLE MICROTISSUES
The ability to examine the contractile and electrophysiological characteristics of mature skeletal muscle is incredibly advantageous in the context of fundamental as well as drug development research. With its two built-in micropillars that serve to form 3D skeletal muscle microtissues in vitro, the OMEGAMP is the ideal platform for the development of next-generation in vitro models of muscular dystrophy (e.g. Duchenne’s, Becker’s) and Kennedy’s disease (SBMA), as well as a powerful platform with which to study muscle regeneration using stem cell technologies.
Every OMEGAMP device contains two chambers, meaning that two independent experiments can be performed with one device.
Diameter: 21.25 mm, round
(specifically designed for 12 well microplates with well diameters of 22 mm)
4 x OMEGAMP devices (shipped sterile)
1 x 12 well microplate
Every OMEGA96-MP device contains 96 chambers, meaning that 96 independent experiments can be performed with one device.
Microplate structure material: Polydimethylsiloxane (PDMS), biologically inert
Microplate outer dimensions: 127.7 x 85.4 mm
Microplate height (w/o lid): 14.2 mm
Chamber pitch: 9 mm
Shipped sterile and ready-to-go
Anti-evaporation tracks around the edge of each plate
Equivalent to 12 OMEGAMP kits, at a fraction of the price