OMEGA

NEURONAL CO-CULTURE DEVICES

The OMEGA series features powerful neuronal co-culture and compartmentalization devices that let you go further!

To increase your data output, we created a unique open chamber design so that each experimental unit consists of only two chambers separated by microfluidic microchannels. Thanks to this streamlined design, OMEGA devices feature more experimental units per area than conventional microfluidic devices for neurons!

To suit all your needs, we continuously grow our neuronal co-culture device portfolio: from two experiments per device all the way to HTS-compatible microplates with 48 experiments ideal for drug screening assays!

Not only did we design this series to increase your data output, but also to make the devices easy to use! That’s why all devices come ready-to-go and pre-bonded!

Check out the TOP ADVANTAGES, APPLICATIONS, FORMATS, and USER GUIDES below!

TOP ADVANTAGES

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PHYSIOLOGICALLY RELEVANT

Neurons depend on their highly polarized structural configuration to properly perform their primary physiological function. Devices of the OMEGA series replicate neuronal polarity in vitro by employing microfabricated channels, which physically separate the two chambers of the experimental unit. Despite their small size, the microchannels are large enough for axons, but not cell bodies, to pass through and project into (and interact with cultures located in) the adjacent chamber creating a more physiological model.

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CHOOSE YOUR FORMAT

If you are just starting out, we recommend using the OMEGA4 device with two experiments per device.
For expensive cell lines, we suggest trying the OMEGA4-2MINI which features one standard-sized chamber and one smaller chamber per experimental unit. Just as for the OMEGA4, each OMEGA4-2MINI lets you set up two experiments per device.
Ready to perform more experiments? Then save money with the HTS-compatible OMEGA96  featuring 48 experiments per microplate!

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START RIGHT AWAY

We know bonding devices can be a real hassle! That's why we ship our OMEGA devices pre-bonded and completely ready-to-go so that you can focus on performing experiments without losing time. OMEGA4 and OMEGA4-2MINI devices are pre-bonded to #1.5 glass coverslips and OMEGA96 plates are pre-bonded to 200µm high-transmissive glass. All devices come sterile in PBS. There is no need for you to use any plasma treatment!

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ACCESS CULTURES DIRECTLY

The unique open chamber design gives you direct access to your cultures! With OMEGA devices, you can explore new types of experimental paradigms not possible with standard closed-chamber devices. For example, chambers are large enough to house tissues that are several millimeters in diameter, easily accomodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids). Additionally, conventional patch-clamp electrodes can openly access the cultures, allowing you to examine the electrophysiological properties of the cultures.

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VERSATILE

One device: so many applications! The OMEGA series offers a plethora of possibilities for experiments involving neuronal co-cultures and compartmentalization. Generate powerful disease models, perform axotomy experiments in vitro, take high quality fluorescence images and record patch-clamp data... Devices are compatible with iPSC-derived cultures, primary cultures, immortalized cell lines and even self-assembled 3D cultures (e.g. spheroids, organoids) and tissue explants can be accommodated!

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AFFORDABLE

Thanks to the streamlined design of only two chambers per experimental unit, the OMEGA series accommodates more experiments per area compared to conventional neuronal co-culture devices! That way, you can increase your data output and save money at the same time! For the most bang for the buck, consider using the OMEGA96 microplate!

OMEGA vs. CONVENTIONAL DEVICES

A QUICK SIDE BY SIDE COMPARISON

APPLICATIONS

NEURONAL COMPARTMENTALIZATION

Neurons seeded in a single chamber of the OMEGA device will project their neurites through the microchannels into the adjacent chamber. Fluidic isolation of one chamber from the other by controlling the direction of the pressure head through the microchannels allows for the fluidic separation of neuronal somas from their projecting axons. Each of these neuronal components can then be treated and analyzed separately.

COMPARTMENTALIZED UPTAKE EXPERIMENTS

Examine compartment-specific uptake, intraneuronal transport, and propagation of toxic or misfolded proteins such as tau, amyloid-β, α-synuclein, TDP43, or prion PrP. The fluidic isolation of compartments with the OMEGA device stops the extracellular travel of applied molecules to the adjacent compartment, providing researchers with the utmost confidence in the interpretation of results.

AXOTOMY

Owing to the open-top chamber design, the OMEGA provides unprecedented access to axons projecting through inter-chamber microchannels. Performing axotomy experiments have never been easier.

CO-CULTURE APPLICATIONS

The OMEGA series is the idea neuronal co-culture system for e.g. motor neurons / skeletal muscle co-cultures. Thanks to the microchannels that separate the two culture chambers, motor neuron somas never come into direct contact with skeletal muscle and you can easily study physiologically relevant neuromuscular junctions. Ideal for neuromuscular disorder applications such as ALS and Kennedy’s disease (SBMA).

 

Other co-culture applications:

Nigrostriatal pathway - Dopaminergic neurons / GABAergic neurons: Replicate this classic dopaminergic pathway in vitro for Parkinson’s disease research.

Nociception and mechanosensation - Co-culture sensory neurons (e.g. DRGs) adjacent to skin fibroblasts to form an excellent pain model.

Myelination - Examine the myelination potential of oligodendrocytes co-cultured in the somatic or axonal neuronal compartments, or both!

Investigate the brain-gut axis by co-culturing vagal, sympathetic or spinal afferent fibers adjacent to gastrointestinal explants or self-assembles gut organoids.

2-IN-1 VIRAL TRANSMISSION ASSAY

The ability to fluidically isolate adjacent compartments with OMEGA devices can be employed to examine the infection and propagation characteristics of viruses in vitro. The OMEGA device allows researchers to access both the processes of viral packaging/release from infected cells as well as viral docking and infection of host cells in one simple experiment.

FORMATS

OMEGA4

Neuronal microfluidic co-culture and compartmentalization device OMEGA-4 by eNUVIO with two experiments per device. Each experimental unit consists of two chambers (chamber #1 filled with blue colorant, chamber #2 filled with red colorant) connected by microfluidic microchannels. This PDMS based device is pre-bonded to a glass coverslip.

Every OMEGA4 device contains two pairs of adjoined chambers, meaning that two independent experiments can be performed with one device.

OMEGA4 specifications:
Glass coverslip: 22 mm, round, #1.5 thickness
Number of microchannels per interface: 70
Microchannel length: ~640 µm
Well surface area: ~0.35cm2

Starter Kit includes:
4 x OMEGA4 devices (shipped sterile)
4 x circular cell culture evaporation minimizers (reusable)
4 x 35 mm round culture dishes (sterile)
1 x Microscopy adapter for OMEGA series devices

Refill Kit includes:
4 x OMEGA4 devices (shipped sterile)
4 x 35 mm round culture dishes (sterile)

SKU: eN-o4-001 (starter kit)
SKU: eN-o4-002 (refill kit)

OMEGA4-2MINI

omega4-2mini Refill Kit

Every OMEGA4-2MINI device contains two pairs of adjoined chambers, meaning that two independent experiments can be performed with one device.

OMEGA4-2MINI specifications:
Glass coverslip: 22 mm, round, #1.5 thickness
Number of microchannels per interface: 70
Microchannel length: ~640 µm
Well #1 surface area: ~0.35cm2
Well #2 surface area: ~0.175cm2

Starter Kit includes:
4 x OMEGA4-2MINI devices (shipped sterile)
4 x circular cell culture evaporation minimizers (reusable)
4 x 35 mm round culture dishes (sterile)
1 x Microscopy adapter for OMEGA series devices

Refill Kit includes:
4 x OMEGA4-2MINI devices (shipped sterile)
4 x 35 mm round culture dishes (sterile)

SKU: eN-o4m2-001 (starter kit)
SKU: eN-o4m2-002 (refill kit)

OMEGA96

Photograph of eNUVIO's OMEGA-96 device, microplate with 48 pairs of chambers filled with red and blue liquid. One red and one blue chamber form a unit.

Every OMEGA96 device contains 48 pairs of adjoined chambers, meaning that 48 independent experiments can be performed with one device.

OMEGA96 specifications:
Microplate structure material: Polydimethylsiloxane (PDMS), biologically inert
Microplate glass or PDMS bottom thickness: 200 µm
Microplate outer dimensions: 127.7 x 85.4 mm
Microplate height (w/o lid): 14.2 mm
Well surface area: ~0.35 cm2
Well dimensions (L x W x H): 7 x 6 x 13 mm
Well volume: 350 µl
Microchannel width: 10 µm
Microchannels/well- pair: 70
Microchannel length: 640 µm
Shipped sterile and ready to be used in culture
Optionally pre-coated with either poly-D-lysine or poly-L-ornithine

SKU: eN-o96-001 (glass bottom, uncoated)
SKU: eN-o96-002 (glass bottom, PLO coated)
SKU: eN-o96-003 (glass bottom, PDL coated)
SKU: eN-o96-007 (PDMS bottom, uncoated)
SKU: eN-o96-008 (PDMS bottom, PLO coated)
SKU: eN-o96-009 (PDMS bottom, PDL coated)

USER GUIDES