EB-DISK
REUSABLE EMBRYOID BODY FORMATION DEVICES
Form countless self-assembled embryoid bodies (EBs) or spheroids with our EB formation devices. We designed the EB-DISKs to facilitate growing large quantities of uniform EBs on a small footprint without requiring much of your time.
The unique microwell design ensures highly reproducible growth of spheroid cultures. EB-DISKs are available in 137, 360 or 948 microwell format so you can easily grow up to 948 EBs in a single culture dish. Best of all? Change the media for all EBs in just a few seconds! Transferring EBs is rapid and gentle too! And if you want, renew and reuse the EB-DISK!
Check out the TOP ADVANTAGES, APPLICATIONS, FORMATS, and USER GUIDES below!
TOP ADVANTAGES
SPACE-SAVING
With the EB-DISKs you can grow up to 948 EBs in a single 60mm dish! If you were to use conventional round bottom microplates instead, you would need 10 plates! Just imagine how much incubator space you can save! Or how many thousands of spheroids you can grow in a single incubator!
TIME-SAVING
Do you think generating EBs is going to be an endless task? With our EB-DISKs you can seed all your cells for up to 948 embryoid bodies in a single step! When you are ready for media changes, you will be able to change it for all EBs at the same time! And finally, when your EBs are ready to be transferred, you will be able to do so in a blink of an eye! No more endless pipetting!
GENTLE
The unique FLIP TO RELEASE transfer method greatly improves the speed and ease of transferring spheroids into larger vessels for further development and growth. It also ensures a much more gentle EB transfer compared to pipetting! Simply flip the EB-DISK over a suitable collection vessel to safely release and transfer as much as 948 spheroids in one shot. No more damaged or lost spheroids due to pipetting! Watch the video below to see how easy it is to transfer EBs!
CHOOSE YOUR FORMAT
We offer EB-DISKs in three different formats so that you can easily grow 137, 360 or 948 embryoid bodies in standard culture dishes. For your convenience, you can further choose between pre-treated EB-DISKs with an ultra-low attachment (ULA) surface coating or follow a simple coating procedure outlined in the user guide to do it yourself. If you want to generate, process and image spheroids without having to ever manipulate or transfer your EBs, our microfluidic HTS-compatible EB-CLARIFY is for you!
REUSABLE
EB-DISKs are made from a biologically inert, durable and flexible polymer, which is autoclavable! Once you’ve released your EBs for further processing or growth, follow the simple cleaning protocol outlined in the EB-DISK user guide and rejuvenate the device. That way, you can reuse your EB-DISKs over and over! Say goodbye to wasting countless plastic microplates to generate embryoid bodies!
AFFORDABLE
Thanks to our proprietary fabrication method and in-house production, we can keep our prices low! If you want, you can get even more out of your EB-DISKs by reusing them. Simply follow the rejuvenation protocol in the user guide.
APPLICATIONS
TRANSFERRING EBs
When your spheroids are ready for the next phase of growth, lift the EB-DISK out of the culture dish, then simply flip the disk to transfer the spheroids safely and efficiently into larger vessels for further growth. This makes for a simpler, gentler and much more rapid transfer of your EBs.
TRANSFERRING EBs
When your spheroids are ready for the next phase of growth, lift the EB-DISK out of the culture dish, then simply flip the disk to transfer the spheroids safely and efficiently into larger vessels for further growth. This makes for a simpler, gentler and much more rapid transfer of your EBs.
VIDEO PROTOCOL
Generating hMOs
Our partners at the Early Drug Discovery Unity of The Neuro developed a method to scale up the generation of hMOs (human midbrain organoids) using our EB-DISK948. This opens up the possibility to generate larger numbers of hMOs, in a manner that minimizes the amount of labor required, while decreasing variability and maintaining the viability of these hMOs over time.
Their video describes the methodology to generate hMOs from a single EB-DISK containing 948 microwells. Click here to access the published version of this protocol by Mohamed et al.!
VIDEO PROTOCOL
Generating hMOs
Our partners at the Early Drug Discovery Unity of The Neuro developed a method to scale up the generation of hMOs (human midbrain organoids) using our EB-DISK948. This opens up the possibility to generate larger numbers of hMOs, in a manner that minimizes the amount of labor required, while decreasing variability and maintaining the viability of these hMOs over time.
Their video describes the methodology to generate hMOs from a single EB-DISK containing 948 microwells. Click here to access the published version of this protocol by Mohamed et al.!
FORMATS
EB-DISK137
Every EB-DISK137 device contains 137 microwells, meaning that the culture media of 137 EBs can be replaced at the same time.
Disk diameter: 19.5 mm (fits the wells of 12-well plates)
Volume of each microwell: 1 mm3
Microwell diameter at base: 900 µm
Kit includes:
4 x EB-DISK137 (shipped sterile)
EB-DISKS are completely reusable
Autoclavable
SKU: eN-eb137-001 (without ULA coating)
SKU: eN-eb137u-001 (with ULA coating)
EB-DISK360
Every EB-DISK360 device contains 360 microwells, meaning that the culture media of 360 EBs can be replaced at the same time.
Disk diameter: 33 mm (fits the wells of 6-well plates)
Volume of each microwell: 1 mm3
Microwell diameter at base: 900 µm
Kit includes:
3 x EB-DISK360 (shipped sterile)
EB-DISKS are completely reusable
Autoclavable
SKU: eN-eb360-001 (without ULA coating)
SKU: eN-eb360u-001 (with ULA coating)
EB-DISK948
Every EB-DISK948 device contains 948 microwells, meaning that the culture media of 948 EBs can be replaced at the same time.
Disk diameter: 52 mm (fits 60 mm round culture dishes)
Volume of each microwell: 1 mm3
Microwell diameter at base: 900 µm
Starter Kit includes:
2 x EB-DISK948 (shipped sterile)
2 x 60 mm culture dish (sterile)
1 x single centrifuge adapter
Refill Kit includes:
1 x EB-DISK948 (shipped sterile)
1 x 60 mm culture dish (sterile)
EB-DISKS are completely reusable
Autoclavable
SKU: eN-eb948-001 (starter kit, without ULA coating)
SKU: eN-eb948u-001 (starter kit, with ULA coating)
SKU: eN-eb948-002 (refill kit, without ULA coating)
SKU: eN-eb948u-002 (refill kit, with ULA coating)
USER GUIDES
