Photograph of eNUVIO's OMEGA AG device with three chambers. Source chamber 1 is filled with blue liquid, source chamber two is filled with red liquid, neuronal chamber is filled with colorless liquid, waste chamber is filled with silver liquid. Device is placed in a 35mm petri dish with a blue evaporation minimizer
Photograph of eNUVIO's OMEGA AG device with three chambers. Source chamber 1 is filled with blue liquid, source chamber two is filled with red liquid, neuronal chamber is filled with colorless liquid, waste chamber is filled with purple liquid.
Photograph of eNUVIO's OMEGA AG device with three chambers, a central viewing area and a waste chamber
Schematic of Omega AG axon guidance device. coloured lines indicating five areas, left: blue source chamber number1, bottom: orange, neuronal chamber, right: magenta source chamber number two, top: green waste chamber, middle: red viewing area. Red arrows pointing from bottom to middle, blue arrows pointing from left to middle and then up, magenta arrows pointing from right to middle and then up, green arrows pointing frim middle up, thin black lines connect the middle area with the outer chambers
Photograph of magnified microfluidic Omega AG axon guidance device. four chambers connect from the left, right, bottom and top to a central chamber via microfluidic channels. Left microchannels are filled with blue fluid, right microchannels are filled red fluid, color gradient in the the central chamber from blue to red, left to right. Chambers are identified as waste chamber (top), source chamber #1 (left), source chamber #2 (right), neuronal chamber (bottom).
Photograph. Sideview of microscopy adapter made from aluminum, rectangular shape with two pins protruding upwards, engraved eNUVIO logo on top right, a round Omega device is placed in the middle, blue acrylic plate attached to pins and placed on top of OMEGA device

OMEGAAG
Axon Guidance and Chemotaxis Device
(3 experiments)

CAD$ 470.00 – CAD$ 520.00

The OMEGAAG device has been designed to help researchers study axon guidance and chemotactic factors in vitro. The device has 4 chambers that are each connected to a central viewing area via a series of microchannels. The flow of fluid across the microchannels can be controlled by simply adjusting the relative fluid volumes in each of the chambers. This asymmetric volume loading of the chambers can serve to fluidically isolate adjacent chambers (like for our OMEGA4 devices), but can also be used to control the direction of flow into and out from the viewing area. Consequently, the viewing area can be a mixing chamber where molecules (or cultures) of interest that have been added to the source chambers will produce a long-lasting and uniform chemical gradient across the viewing area (see photo – blue and red chambers).

In this way, the OMEGAAG is well suited to perform axon guidance experiments such as growth cone turning assays. Neurons that have been seeded in the neuronal chamber (see photo – transparent chamber) will project axons through the microchannels into the viewing area where they are exposed to a gradient of chemotactic factors. The growth cones can then be simply monitored for turning behavior. During these experiments, it may be necessary to isolate the neuronal chamber (somatic components) from the viewing area, to prevent the somatic components from being exposed to the chemotactic factors. This is achieved by maintaining a relatively higher volume of fluid in the neuronal chamber, and by reducing the volume of fluid in the waste chamber (see photo – purple chamber). Doing so promotes the overall flow of fluid from the viewing chamber towards the waste chamber, and not into the neuronal chamber.

As with all OMEGA series devices, the OMEGAAG has open tops, giving researchers the ability to use explants or self-assembled 3D cultures (e.g. spheroids, organoids) in addition to primary or iPSC-derived neurons in their experiments. This means that even the source chambers can be used for culturing cells or tissues – allowing scientists to get creative with new types of growth cone turning assays that might involve explanted tissue, for example. The devices come ready to use in culture – they are sterile, hydrophilic and pre-bonded to #1.5 glass coverslips.

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Description

Culture compatibility:

  • iPSC-derived myogenic/neurgenic precursors
  • Primary cells from embryonic sources or biopsies
  • Explants
  • Self-assembled 3D cultures (e.g. spheroids or organoids)
  • Immortalized myogenic cell lines

OMEGAAG Specifications:

  • Chamber working volume: 30 – 100 µL
  • Viewing area surface area: 1 mm2
  • Number of interfaces: 4
  • Number of microchannels per interface: 20
  • Pre-bonded glass base (#1.5 thickness)
  • Glass coverslip diameter: 22 mm

The OMEGAAG Starter kit includes:

  • 3 x OMEGAAG devices
  • 3 x circular cell culture evaporation minimizers (reusable)
  • 3 x 35 mm round culture dishes (sterile)
  • 1 x 35 mm microscope stage adapter (reusable)

The OMEGAAG Refill Kit includes:

  • 3 x OMEGAAG devices
  • 3 x 35 mm round culture dishes (sterile)

Additional information

Weight 0.9 kg
Dimensions 25.4 × 17.8 × 7.6 cm
Format

Starter Kit, Refill Kit

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