Our OMEGANMJ neuromuscular junction device merges the simplicity of microfluidics with the power of 3D cell culture to help you form advanced in vitro neuromuscular junction models. Easily create human-relevant neuromuscular disease models (e.g., Duchenne-type Muscular Dystrophy (DMD), Kennedy’s disease (SBMA), Amyotrophic Lateral Sclerosis (ALS)). Our NMJ-on-a-chip™ technology offers you a truly unique and time-saving tool.
To form muscle neuron co-cultures, resuspend myogenic progenitor cells from primary, iPSC or immortalized sources in an ECM-derived hydrogel mixture, and seed them into the muscle culture chamber. After polymerization, the hydrogel-suspended myoblasts begin to self-assemble into a 3D skeletal muscle microtissue using the two built-in micropillars as anchors. Once differentiated and matured, these muscle microtissues twitch and contract as is expected from skeletal muscle. This allows for the examination of the contractile and electrophysiological characteristics of mature skeletal muscle in vitro. Motor neurons seeded in the neuronal chamber will project axons through the microchannels into the muscle chamber, where they will interact with the established 3D skeletal muscle microtissue.
As with all eNUVIO’s OMEGA series, the OMEGANMJ has open-top chambers, giving you the ability to use explants or self-assembled 3D cultures (e.g. spheroids, organoids) in addition to primary or iPSC-derived neurons in your experiments. Each device contains two adjoined pairs of chambers, so two separate experiments can be performed with one device. The devices come ready-to-use in culture – they are sterile, hydrophilic and are pre-bonded to a high-transmissive PDMS base.
Culture compatibility:
- iPSC-derived myogenic/neurgenic precursors
- Primary cells from embryonic sources or biopsies
- Explants
- Self-assembled 3D cultures (e.g. spheroids or organoids)
- Immortalized myogenic cell lines
OMEGANMJ specifications:
- High-transmissive PDMS base: 22 mm round
The OMEGANMJ Starter Kit includes:
- 3 x OMEGANMJ devices
- 3 x circular cell culture evaporation minimizers (reusable)
- 3 x 35 mm round culture dishes (sterile)
- 1 x 35 mm microscope stage adapter (reusable)
The OMEGANMJ Refill Kit includes:
- 3 x OMEGANMJ devices
- 3 x 35 mm round culture dishes (sterile)
The OMEGANMJ Refill Kit Plus includes:
- 3 x OMEGANMJ devices
- 3 x circular cell culture evaporation minimizers (reusable)
- 3 x 35 mm round culture dishes (sterile)
Learn more about the TOP ADVANTAGES and APPLICATIONS of the OMEGANMJ series.
