OMEGANMJ
Neuromuscular Junction Device
(6 experiments)

CAD$ 470.00CAD$ 520.00

3 x OMEGANMJ devices, 6 experiments!

The OMEGANMJ device has been specifically designed to recapitulate neuromuscular synaptic physiology in vitro. Motor neurons seeded in the neuronal culture chamber extend axons into the adjoined oval muscle chamber through a series of microchannels that fluidically isolate the two culture chambers. This way, the somatic motor neuron components are never directly exposed to the muscle tissue they innervate, forming a system that more closely resembles the native neuromuscular physiology. The OMEGANMJ is the ideal platform for the development of next-generation models of neuromuscular disorders such as Duchenne-type muscular dystrophy (DMD), Kennedy’s disease (SBMA) as well as ALS.

The adjoined muscle culture chamber contains two micropillars that form anchor points for the formation of 3D skeletal muscle tissue. Compared to monolayer cultures, these 3D cultures are more stable for long-term culture applications and mature rapidly and more completely, making 3D skeletal muscle cultures ideal for establishing long-term motoneuron/muscle co-cultures.

Looking to form 3D skeletal muscle microtissues only? Check out our OMEGAMP device here.

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Description

The OMEGANMJ device merges the simplicity of microfluidics with the power of 3D cell culture to form one of the most advanced in vitro experimental models of neuromuscular disorders.

Myogenic progenitor cells from primary, iPSC or immortalized sources are resuspended in an ECM-derived hydrogel mixture, and are seeded into the muscle culture chamber. After polymerization, the hydrogel-suspended myoblasts begin to self-assemble into a 3D skeletal muscle microtissue using the two built-in micropillars as anchors. Once differentiated and matured, these muscle microtissues twitch and contract as is expected from skeletal muscle. This allows for the examination of the contractile and electrophysiological characteristics of mature skeletal muscle in vitro.

Motor neurons seeded in the neuronal chamber will project axons through the microchannels into the muscle chamber, where they will interact with the established 3D skeletal muscle microtissue.

As with all eNUVIO’s OMEGA series, the OMEGANMJ has open-top chambers, giving researchers the ability to use explants or self-assembled 3D cultures (e.g. spheroids, organoids) in addition to primary or iPSC-derived neurons in their experiments. Each device contains two adjoined pairs of chambers, so two separate experiments can be performed with one device. The devices come ready-to-use in culture – they are sterile, hydrophilic and are pre-bonded to a high-transmissive PDMS base.

Culture compatibility:

  • iPSC-derived myogenic/neurgenic precursors
  • Primary cells from embryonic sources or biopsies
  • Explants
  • Self-assembled 3D cultures (e.g. spheroids or organoids)
  • Immortalized myogenic cell lines

OMEGANMJ specifications:

  • High-transmissive PDMS base: 22 mm round

The OMEGANMJ Starter kit includes:

  • 3 x OMEGANMJ devices
  • 3 x circular cell culture evaporation minimizers (reusable)
  • 3 x 35 mm round culture dishes (sterile)
  • 1 x 35 mm microscope stage adapter (reusable)

The OMEGANMJ Refill kit includes:

  • 3 x OMEGANMJ devices
  • 3 x 35 mm round culture dishes (sterile)

Additional information

Weight 0.9 kg
Dimensions 25.4 × 17.8 × 7.6 cm
Format

Starter Kit, Refill Kit

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