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Scientist holding HTS-compatible microfluidic neuronal co-culture and compartmentalization screening plate OMEGA-96 microplate by eNUVIO.
eNUVIO's OMEGA series versus conventional neuronal co-culture and compartmentalization devices for web shop
Photograph of eNUVIO's OMEGA-96 device, microplate with 48 pairs of chambers filled with red and blue liquid. One red and one blue chamber form a unit.
Microplate specifications for eNUVIO’s OMEGA-96 neuronal microfluidic co-culture and compartmentalization screening plate
iPSC-derived motor neurons progenitor cells were seeded in the left chamber of eNUVIO's OMEGA neuronal microfluidic co-culture and compartmentalization device. The neurons have extended their axons through the microchannels into the right chamber. After 14 DIV, the culture was fixed and immunolabeled with an anti-MAP2 antibody (magenta). Image courtesy of Maria Castellanos Montiel and Dr. Thomas Durcan, The Neuron - McGill University
Mesencephalic cultures were established on a glia feeder layer in both chambers of eNUVIO's OMEGA neuronal microfluidic co-culture and compartmentalization device. After 21 DIV, the culture was fixed and labelled with DAPI (blue), anti-tyrosine hydroxylase antibody (TH; green; dopaminergic neuron marker), and anti-MAP2 antibody (red). Image courtesy of Samuel Burke-Nanni and Dr. Louis- Éric Trudeau, Université de Montréal
Motor neuron/skeletal muscle co-cultured in eNUVIO's OMEGA neuronal microfluidic co-culture and compartmentalization device. iPSC-derived motor neurons seeded in the left chamber extended their axons through the microchannels into the right chamber containing differentiated skeletal muscle. Cultures were fixed at 14 DIV, and labeled with anti-SV2 antibody (red), fluorescent bungarotoxin (green) and Hoechst (blue). Image courtesy of Maria Castellanos Montiel and Dr. Thomas Durcan, The Neuro - McGill University
iPSC-derived dopaminergic neurons were established in the right chamber of eNUVIO's OMEGA neuronal microfluidic co-culture and compartmentalization device and have projected axons through the microchannels into the left chamber. After 14 DIV, the culture was labeled using a plasma membrane marker (green) and fixed before imaging. Image courtesy of Drs. Aurélie de Rus Jacquet and Francesca Cicchetti, CHU de Québec - Université Laval
Primary neuronal cultures were established in both chambers of eNUVIO's OMEGA neuronal microfluidic co-culture and compartmentalization device. Neurons project their axons across the microchannels in both directions. As the left chamber is kept fluidically isolated from the right chamber, unlabeled alpha-synuclein PFFs are added to the left chamber only. After 10 DIV, cultures were fixed and immunolabeled against MAP2 (blue) and pSyn (red). Image courtesy of Drs. Lenka Schorova and Simon Wing, McGill University Health Center
Human iPSC-derived motor neuron progenitor cells were seeded in the left chamber of the OMEGA device by eNUVIO. The neurons extended their axons through the microchannels into the right chamber. After 7 days in culture, mechanical axotomy was performed in the right chamber using a pipette tip to axotomize the projected axons. The culture was allowed to recover for another 7 days before being fixed and immunolabeled with anti-TUJ1 (green).

OMEGA96
Neuronal Co-Culture Microplate

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1 x OMEGA96 screening plate, 48 experiments! Equivalent to 24 OMEGA4 or 48 devices from competitors, at a fraction of the price!

The first of its kind. It is the scaled up version of the OMEGA4, High-Throughput Screening (HTS) Compatible.

Speed and scalability are the key to rapidly identifying new therapeutics. Owing to the unique open-top chamber design, the OMEGA96 plate is the ONLY microfluidic platform commercially available that fully conforms to the 96-well microplate standard. As such, the OMEGA96 plate is compatible with most lab automation and robotic systems such as microplate washers, plate readers, high-content imagers, pinning tools and plate hotels.

The OMEGA96 screening plate opens up the way to so many assays. In a basic application, the plate serves as a compartmentalization tool to isolate the somatic and axonal components of seeded neurons. This clarifies the results from toxic species uptake assays and makes glutamate toxicity assays a breeze to setup. Used as a co-culture device,  the OMEGA96 is an ideal platform to study neurodegeneration, the neuromuscular junction (NMJ), the nigrostriatal pathway, pain pathways, myelination, as well as chemotactic-releasing cultures.

OMEGA96 plate features:

  • Ready-to-use: sterile and pre-bonded to a high-transmissive glass coverslip
  • Optionally  pre-coated with either poly-D-lysine or poly-L-ornithine
  • 48 experiments can be performed per plate – perfect for screening!
  • Open-top chamber design provides full access to your cultures – use explants or spheroids in your experiments

Just starting out? Then you might want to try our OMEGA4 device first.

Need a third culture chamber? The OMEGA96-ACE might be what you are looking for!

Download the USER GUIDE here!

Learn more about the TOP ADVANTAGES, APPLICATIONS and other FORMATS of the OMEGA series.

SKU: eN-o96-001, eN-o96-002, eN-o96-003 Categories: , Tags: , , , , , , ,

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High-Throughput Screening Compatible

The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the chambers of the device are physically separated by a series of microfluidic channels. The small size of the microchannels serve to fluidically isolate each of the adjoined chambers, but are large enough to allow neurites/axons to project into and interact with cultures located in the adjacent chamber.

The unique open-chamber design of the OMEGA gives scientists direct access to their co-cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, easily accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids).

OMEGA96 plates are shipped ready to use. They are sterile, pre-bonded to high-transmissive glass, and optionally pre-coated with either poly-D-lysine or poly-L-ornithine. Maintaining healthy cultures over a long period of time is even easier with OMEGA plates, owing to the evaporation-minimizing tracks around the edge of each plate. 

 

OMEGA96 devices are suited for in vitro co-culture studies involving:

  • Neuromuscular junction (NMJ) in the context of ALS, Kennedy’s disease (SBMA), Duchenne’s muscular dystrophy
    • e.g. motor neuron/muscle co-culture
  • Nigrostriatal pathway in the context of Parkinson’s disease
    • e.g. dopaminergic neuron (SNc)/GABAergic neuron (striatal) co-culture
  • Sensory system in the context of nociception and mechanosensation
    • e.g. skin fibroblast/DRG co-culture
  • Myelination
    • e.g. neuron/oligodendrocyte or Schwann cell co-culture
  • Enteric nervous system
    • e.g. enteroendocrine cells/vagal neuron co-culture

Culture compatibility:

  • iPSC-derived cultures
  • Self-assembled 3D cultures (e.g. spheroids, organoids)
  • Primary cultures
  • Tissue explants
  • Immortalized Cell lines

OMEGA96 specifications:

  • 48 well-pairs per microplate = 48 individual experiments per plate
  • LxWxH: 127.2 x 85.3 x 14.1 mm
  • Chamber surface area: ~0.35 cm2
  • Chamber diameter: 6 mm
  • Chamber volume: 350 μL
  • Chamber pitch: 9 mm
  • Microchannel width: 10 μm
  • Microchannel length: ~640 μm
  • Microchannels/chamber pair: 70
  • Pre-bonded glass bottom: 200 μm thick
  • Shipped sterile in PBS, ready to use
  • Hydrophilic, no need for plasma treatment
  • Option: pre-coated with poly-D-lysine or poly-L-ornithine
  • Anti-evaporation tracks around the edge of each plate

 

Learn more about the TOP ADVANTAGES, APPLICATIONS and other FORMATS of the OMEGA series.

Weight 0.1 kg
Dimensions 13 × 9 × 2 cm
Bottom material

Glass, PDMS
Coating

No coating, Poly-L-ornithine, Poly-D-lysine

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