{"id":2086,"date":"2020-10-26T15:30:53","date_gmt":"2020-10-26T19:30:53","guid":{"rendered":"https:\/\/enuvio.com\/?page_id=2086"},"modified":"2024-03-04T17:42:47","modified_gmt":"2024-03-04T22:42:47","slug":"triple-chamber-neuronal-co-culture-devices","status":"publish","type":"page","link":"https:\/\/enuvio.com\/triple-chamber-neuronal-co-culture-devices\/","title":{"rendered":"OMEGA ACE"},"content":{"rendered":"
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\n\t\t\t\t\t\tOMEGAACE<\/sup>\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t\t\t\t\t\t\t

\n\t\t\t\t\t\tTRIPLE-CHAMBER NEURONAL CO-CULTURE DEVICES\t\t\t\t\t\t<\/h1>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>
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      The two chambers of the OMEGA device<\/strong><\/a> aren\u2019t enough for your in vitro assay? For more flexibility, we designed the OMEGA<\/b>ACE<\/b><\/sup> triple chamber neuronal co-culture and compartmentalization<\/b> device series!<\/span><\/p>\n

      Each experimental unit features three serially-adjoined culture chambers that are physically separated by microfluidic microchannels<\/b>.<\/span><\/p>\n

      Choose between different chamber sizes and device formats: from a single experiment per device all the way to HTS-compatible<\/b> microplates with 32 experiments ideal for drug screening assays<\/b>. We\u2019ve got you covered!<\/span><\/p>\n

      Of course, all OMEGAACE<\/sup> devices have open chambers and come pre-bonded to glass and ready-to-go so you can start your experiments right away!<\/span><\/p>\n

      Check out the TOP ADVANTAGES<\/a>, APPLICATIONS<\/a>, FORMATS<\/a>, and USER GUIDES<\/a> below!<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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      \n\t\t\t\t\t\tTOP ADVANTAGES\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>
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      \n\t\t\"physiologically\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tPHYSIOLOGICALLY RELEVANT\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      Neurons depend on their highly polarized structural configuration<\/b> to properly perform their primary physiological function. Devices of the OMEGAACE<\/sup> series replicate neuronal polarity in vitro<\/i> by employing microfabricated channels<\/b>, which physically separate the three chambers<\/b> of the experimental unit. Despite their small size, the microchannels are large enough for axons<\/b>, but not cell bodies, to pass through<\/b> and project into (and interact with cultures located in) the adjacent chambers creating a more physiological in vitro model.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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      \n\t\t\"icon\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tCHOOSE YOUR FORMAT\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      Depending on your needs, you can choose between four different configurations: the OMEGAACE<\/sup><\/a> with a 250\u00b5m wide middle chamber, the OMEGAACE-2mm<\/sup><\/a> with a 2mm wide middle chamber,\u00a0 the OMEGAACE-4mm<\/sup><\/a> with a 4mm wide fully accessible open-top middle chamber and the OMEGA96-ACE<\/sup><\/a> with a 8mm wide fully accessible open-top middle chamber. You can perform one experiment per device with the OMEGAACE<\/sup><\/a> and OMEGAACE-2mm<\/sup><\/a> and two with the OMEGAACE-4mm<\/sup><\/a>. For drug-screening assays, we suggest using the HTS-compatible OMEGA96-ACE<\/sup><\/a> featuring 32 experiments per microplate!<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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      \n\t\t\"minimalist\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tSTART RIGHT AWAY\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      We know bonding devices can be a real hassle! That's why we ship all of our triple chamber co-culture devices pre-bonded<\/b> and completely ready-to-go<\/b> so that you can focus on performing experiments without losing time. OMEGAACE<\/sup><\/a>,\u00a0OMEGAACE-2mm<\/sup><\/a> and OMEGAACE-4mm<\/sup><\/a> devices are pre-bonded to #1.5 glass coverslips and OMEGA96-ACE<\/sup><\/a> microplates are pre-bonded to 200\u00b5m high-transmissive glass. All devices come sterile in PBS<\/b>. There is no need for you to use any plasma treatment!<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>

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      \n\t\t\"icon\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tACCESS CULTURES DIRECTLY\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      The unique open chamber design<\/b> gives you direct access to your cultures! With devices of the OMEGAACE<\/sup> series, you can explore new types of experimental paradigms not possible with standard closed-chamber devices. For example, chambers are large enough to house tissues that are several millimeters in diameter, easily accommodating tissue explants<\/b> or self-assembled 3D cultures<\/b> (e.g. spheroids, organoids). Additionally, conventional patch-clamp<\/b> electrodes can openly access the cultures, allowing you to examine the electrophysiological properties of the cultures. Note that the 250\u00b5m and 2mm wide middle chambers aren't open-top chambers.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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      \n\t\t\"minimalist\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tVERSATILE\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      The OMEGAACE<\/sup> devices are perfectly suited for in vitro triple co-culture studies<\/strong> of neurodegeneration, neuromuscular junction (NMJ), nigrostriatal pathway, pain, myelination, or chemotactic factors. In single culture applications, the OMEGAACE<\/sup> can serve as a compartmentalization<\/strong> tool to separate the somatic, proximal axonal and distal axonal components<\/strong> of neurons. The wider middle chamber of the OMEGA96-ACE<\/sup><\/a> gives you the highest confidence for interpreting neuronal propagation results<\/strong>! All devices are compatible with iPSC-derived cultures, primary cultures, immortalized cell lines, self-assembled 3D cultures<\/strong> (e.g. spheroids, organoids), tissue explants and you can even perform patch-clamp<\/strong> experiments!<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div>

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      \n\t\t\"affordable\n\t<\/div>\n\n<\/div><\/div>
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      \n\t\t\t\t\t\tAFFORDABLE\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div>
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      Thanks to our proprietary fabrication method and in-house production, we can keep our prices low! Get everything you need with our starter kits<\/strong> and save money later with our refill kits<\/strong>! For triple chamber co-culture devices, the OMEGA96-ACE<\/sup><\/a> offers the most advantageous price per experiment<\/strong>! This way, you can massively increase your data output<\/strong> and save money<\/b> at the same time!<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

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      \n\t\t\t\t\t\tAPPLICATIONS\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>
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      1. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t
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        \n\t\t\t\t\t\tTRIPLE CO-CULTURE\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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        <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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        In triple co-culture applications, each chamber can be used to culture a distinct cell type. For example, motor neurons, oligodendrocytes and skeletal muscle can be co-cultured in the context of neurodegeneration (ALS, Kennedy's disease (SBMA), Duchenne's muscular dystrophy). Each cell type is grown in a separate compartment, connected only via microchannels for fluidic isolation.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

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        \n\t\t\t\t\t\tTRIPLE CO-CULTURE\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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      2. \n\t\t\t\t\t\t\t\t\t\t\n\t\t\t\"Schematic<\/a>\t\t<\/li>\n\t\t<\/ul>\t\t\t\t
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        1. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t
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          In triple co-culture applications, each chamber can be used to culture a distinct cell type. For example, motor neurons, oligodendrocytes and skeletal muscle can be co-cultured in the context of neurodegeneration (ALS, Kennedy's disease (SBMA), Duchenne's muscular dystrophy). Each cell type is grown in a separate compartment, connected only via microchannels for fluidic isolation.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

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            \n\t\t\t\t\t\tNEURONAL COMPARTMENTALIZATION\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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            <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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            In the context of compartmentalization, the extra chamber is especially useful for fluidically separating neurons into three components: somatic, proximal axonal and distal axonal (growth cone). The separated chambers can then be used to apply molecules of interest or perform axotomy.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

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            \n\t\t\t\t\t\tNEURONAL COMPARTMENTALIZATION\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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            1. <\/a><\/li>\n\t\t\t\t\t\t\t\t\t<\/ol>\n\n\t\t\t\t
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              In the context of compartmentalization, the extra chamber is especially useful for fluidically separating neurons into three components: somatic, proximal axonal and distal axonal (growth cone). The separated chambers can then be used to apply molecules of interest or perform axotomy.<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

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              \n\t\t\t\t\t\tFORMATS\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>
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              \n\t\t\t\t\t\tOMEGAACE<\/sup>\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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              <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"Triple\n\t<\/a><\/div>\n\n<\/div><\/div>
              \n\t\n\t\t\n\t\t\t\n\t\t\tSHOP\t\t<\/span>\n\t<\/a>\n<\/div>\n<\/div><\/div>
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              Every OMEGAACE<\/sup> device contains three <\/strong>chambers that are serially adjoined via a series of microfluidic channels. One experiment can be performed per device.<\/span><\/p>\n

              OMEGAACE<\/sup> specifications:<\/strong>
              \nGlass coverslip: 22 mm, round, #1.5 thickness
              \nChamber #2 channel width: 0.25 mm
              \nChamber #2 channel height: 0.5 mm
              \nNumber of microchannels per interface: 70
              \nNumber of interfaces per device: 2
              \nMicrochannel length: >630 \u00b5m<\/span><\/p>\n

              Starter Kit includes:<\/strong>
              \n4 x OMEGAACE<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n1 x Microscopy adapter for OMEGA series devices
              \n(SKU: eN-oace-001)<\/span>
              \n<\/span><\/p>\n

              Refill Kit includes:<\/strong>
              \n4 x OMEGAACE<\/sup> devices (shipped sterile)
              \n4 x 35 mm round culture dishes (sterile)
              \n<\/span>(SKU: eN-oace-002)<\/span><\/p>\n

              Refill Kit Plus includes:<\/strong>
              \n4 x OMEGAACE<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n(SKU: eN-oace-003)<\/span><\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>

              \n\t\t\t\t\t\t\t

              \n\t\t\t\t\t\tOMEGAACE-2mm<\/sup>\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
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              <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
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              \n\t\t\t\n\t\t\t\"Triple\n\t<\/a><\/div>\n\n<\/div><\/div>
              \n\t\n\t\t\n\t\t\t\n\t\t\tSHOP\t\t<\/span>\n\t<\/a>\n<\/div>\n<\/div><\/div>
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              Every OMEGAACE-2mm<\/sup> device contains three <\/strong>chambers that are serially adjoined via a series of microfluidic channels. One experiment can be performed per device.<\/span><\/p>\n

              OMEGAACE-2mm<\/sup> specifications:<\/strong>
              \nGlass coverslip: 22 mm, round, #1.5 thickness
              \nChamber #2 channel width: 2 mm
              \nChamber #2 channel height: 2 mm
              \nNumber of microchannels per interface: 70
              \nNumber of interfaces per device: 2
              \nMicrochannel length: >630 \u00b5m<\/span><\/p>\n

              Starter Kit includes:<\/strong>
              \n4 x OMEGAACE-2mm<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n1 x Microscopy adapter for OMEGA series devices
              \n(SKU: eN-oace2-001)<\/span>
              \n<\/span><\/p>\n

              Refill Kit includes:<\/strong>
              \n4 x OMEGAACE-2mm<\/sup> devices (shipped sterile)
              \n4 x 35 mm round culture dishes (sterile)
              \n<\/span>(SKU: eN-oace2-002)<\/span><\/p>\n

              Refill Kit Plus includes:<\/strong>
              \n4 x OMEGAACE-2mm<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n(SKU: eN-oace2-003)<\/span><\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>

              \n\t\t\t\t\t\t\t

              \n\t\t\t\t\t\tOMEGAACE-4mm<\/sup>\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
              \n\t\t\t\t\t\t
              <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"Triple\n\t<\/a><\/div>\n\n<\/div><\/div>
              \n\t\n\t\t\n\t\t\t\n\t\t\tSHOP\t\t<\/span>\n\t<\/a>\n<\/div>\n<\/div><\/div>
              \n
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              Every OMEGAACE-4mm<\/sup> device contains three <\/strong> fully accessible chambers that are serially adjoined via a series of microfluidic channels. Two experiments can be performed per device.<\/span><\/p>\n

              OMEGAACE-4mm<\/sup> specifications:<\/strong>
              \nGlass coverslip: 22 mm, round, #1.5 thickness
              \nChamber #2 width: 4mm
              \n<\/span>Chamber #1 and #3 width: 4.5mm
              \nAll chambers are fully accessible
              \n<\/span>Number of microchannels per interface: 70
              \nNumber of interfaces per experiment: 2
              \nMicrochannel length: >630 \u00b5m<\/span><\/p>\n

              Starter Kit includes:<\/strong>
              \n4 x OMEGAACE-4mm<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n1 x Microscopy adapter for OMEGA series devices
              \n(SKU: eN-oace4mm-001)<\/span>
              \n<\/span><\/p>\n

              Refill Kit includes:<\/strong>
              \n4 x OMEGAACE-4mm<\/sup> devices (shipped sterile)
              \n4 x 35 mm round culture dishes (sterile)
              \n<\/span>(SKU: eN-oace4mm-002)<\/span><\/p>\n

              Refill Kit Plus includes:<\/strong>
              \n4 x OMEGAACE-4mm<\/sup> devices (shipped sterile)
              \n4 x evaporation minimizing culture inserts (reusable)
              \n4 x 35 mm round culture dishes (sterile)
              \n(SKU: eN-oace4mm-003)<\/span><\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>

              <\/div><\/div>
              \n\t\t\t\t\t\t\t

              \n\t\t\t\t\t\tOMEGA96-ACE<\/sup>\t\t\t\t\t\t<\/h3>\n\t\t\t\t\t\t\t\t\t\t\t
              \n\t\t\t\t\t\t
              <\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"Photo\n\t<\/a><\/div>\n\n<\/div><\/div>
              \n\t\n\t\t\n\t\t\t\n\t\t\tSHOP\t\t<\/span>\n\t<\/a>\n<\/div>\n<\/div><\/div>
              \n
              \n\t

              Every OMEGA96-ACE<\/sup> device contains 32 pairs<\/strong> of adjoined chambers, meaning that 32 independent experiments<\/strong> can be performed with one device.<\/span><\/p>\n

              OMEGA96-ACE<\/sup> specifications:<\/strong>
              \nMicroplate structure material: Polydimethylsiloxane (PDMS), biologically inert
              \nMicroplate outer dimensions: 127.7 x 85.4 mm
              \nMicroplate height (w\/o lid): 14.2 mm
              \nChamber #1 and #3 dimensions (L x W x H): 7 x 6 x 13 mm
              \nChamber #2 dimensions (L x W x H): 8 x 6 x 13 mm
              \nMicrochannel width: 10 \u00b5m
              \nMicrochannels\/well-pair: 70
              \nMicrochannel length: 640 \u00b5m
              \nMicroplate glass or PDMS bottom thickness: 200 \u00b5m
              \nShipped sterile and ready to be used in culture
              \nOptionally pre-coated with either poly-D-lysine or poly-L-ornithine
              \n<\/span><\/p>\n

              SKU: eN-o96ace-001 (glass bottom, uncoated)
              \nSKU: eN-o96ace-002 (glass bottom, PLO coated)
              \nSKU: eN-o96ace-003 (glass bottom, PDL coated)
              \n<\/span><\/p>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>

              <\/div><\/div><\/div><\/div>
              \n\t\t\t\t\t\t\t

              \n\t\t\t\t\t\tUSER GUIDE\t\t\t\t\t\t<\/h2>\n\t\t\t\t\t\t<\/div>\n<\/div><\/div><\/div><\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"thumbnail\n\t<\/a><\/div>\n\n<\/div><\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"thumbnail\n\t<\/a><\/div>\n\n<\/div><\/div><\/div><\/div>
              <\/div><\/div><\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"thumbnail\n\t<\/a><\/div>\n\n<\/div><\/div>
              \n\n
              \n\t\t\t\n\t\t\t\"thumbnail\n\t<\/a><\/div>\n\n<\/div><\/div><\/div><\/div><\/div><\/div><\/div>","protected":false},"excerpt":{"rendered":"

              The two chambers of the OMEGA device aren\u2019t enough for your in vitro assay? For more flexibility, we designed the OMEGAACE triple chamber neuronal co-culture and compartmentalization device series! Each experimental unit features three serially-adjoined culture chambers that are physically separated by microfluidic microchannels. Choose between different chamber sizes and device formats: from a single… Read More »OMEGA ACE<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":7610,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"neve_meta_sidebar":"","neve_meta_container":"","neve_meta_enable_content_width":"off","neve_meta_content_width":100,"neve_meta_title_alignment":"","neve_meta_author_avatar":"","neve_post_elements_order":"","neve_meta_disable_header":"","neve_meta_disable_footer":"","neve_meta_disable_title":"","neve_meta_reading_time":"","footnotes":""},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/pages\/2086"}],"collection":[{"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/comments?post=2086"}],"version-history":[{"count":2,"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/pages\/2086\/revisions"}],"predecessor-version":[{"id":12889,"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/pages\/2086\/revisions\/12889"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/media\/7610"}],"wp:attachment":[{"href":"https:\/\/enuvio.com\/wp-json\/wp\/v2\/media?parent=2086"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}