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Triple-Chamber Neuronal Co-Culture Microplate

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1 x OMEGA96-ACE screening plate, 32 experiments! Equivalent to 32 OMEGAACE or OMEGAACE-2mm devices, at a fraction of the price!

The first of its kind. It is the scaled up version of the OMEGAACE and High-Throughput Screening (HTS) compatible.

Speed and scalability are the key to rapidly identifying new therapeutics. Owing to the unique open-top chamber design, the OMEGA96-ACE plate is the ONLY triple-chamber microfluidic platform commercially available that fully conforms to the 96-well microplate standard. As such, the OMEGA96-ACE plate is compatible with most lab automation and robotic systems such as microplate washers, plate readers, high-content imagers, pinning tools and plate hotels.

OMEGA96-ACE screening plate can be used for in vitro triple co-cultures and studying neuronal propagation.

OMEGA96-ACE plate features:

  • Ready-to-use: sterile and pre-bonded to a high-transmissive glass coverslip
  • Optionally  pre-coated with either poly-D-lysine or poly-L-ornithine
  • 32 experiments can be performed per plate – perfect for screening!
  • Open-top chamber design provides full access to your cultures – use explants or spheroids in your experiment

Just starting out? Then you might want to try our OMEGAACE, OMEGAACE-2mm or OMEGAACE-4mm device first.

Need only two culture chambers? The OMEGA96 might be what you are looking for!

Learn more about the TOP ADVANTAGES, APPLICATIONS and other FORMATS of the OMEGAACE series.

The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the growth chambers of the device are physically separated by a series of microfluidic channels that serve to fluidically isolate each of the adjoined chambers. These microchannels are large enough however, to allow for neurites/axons to project into and interact with cultures located in the adjacent chamber. OMEGA96-ACE devices each contain three serially-adjoined culture chambers. Compared to our two-chamber devices, triple-chamber devices provide researchers with the ability to perform more specialized co-culture and compartmentalization experiments.

The unique open-chamber design of OMEGA series devices gives scientists direct access to their cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids). Additionally, conventional patch-clamp electrodes can openly access the cultures, allowing researchers to examine the electrophysiological properties of the culture.

OMEGA96-ACE plates are shipped ready to use. They are sterile, pre-bonded to high-transmissive glass, and optionally pre-coated with either poly-D-lysine or poly-L-ornithine. Maintaining healthy cultures over a long period of time is even easier with OMEGA96-ACE plates, owing to the evaporation-minimizing tracks around the edge of each plate

OMEGA96-ACE devices can be used to set up in vitro triple co-cultures and study neuronal propagation.


Culture compatibility:

  • iPSC-derived cultures
  • Self-assembled 3D cultures (e.g. spheroids, organoids)
  • Primary cultures
  • Explant tissues
  • Immortalized cell lines

OMEGA96-ACE specifications:

  • 32 well-pairs per microplate = 32 individual experiments per plate
  • Microplate dimensions (L x W x H): 127.2 x 85.3 x 14.1 mm
  • Chamber #1 and #3 dimensions (L x W x H): 7 x 6 x 13 mm
  • Chamber #2 dimensions (L x W x H): 8 x 6 x 13 mm
  • Chamber pitch: 9 mm
  • Microchannel width: 10 μm
  • Microchannel length: ~640 μm
  • Microchannels/chamber pair: 70
  • Pre-bonded glass bottom: 200 μm thick
  • Shipped sterile in PBS, ready to use
  • Hydrophilic, no need for plasma treatment
  • Option: pre-coated with poly-D-lysine or poly-L-ornithine
  • Anti-evaporation tracks around the edge of each plate


Learn more about the TOP ADVANTAGES, APPLICATIONS and other FORMATS of the OMEGAACE series.

Weight 0.9 kg
Dimensions 14 × 17 × 6 cm

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